TECH OFFER

A Surface Acoustic Wave Biosensor Based Point-of-care System for Influenza Diagnosis

KEY INFORMATION

TECHNOLOGY READINESS LEVEL (TRL):
LOCATION:
Singapore
ID NUMBER:
TO174208

TECHNOLOGY OVERVIEW

The point-of-care (POC) system developed is based on a Surface Acoustic Wave (SAW) biosensor to bind and detect hemagglutinin (HA) of Influenza A H1N1 virus subtype. Based on laboratory evaluation, the POC system is capable of detecting HA antigen with sensitivity down to 1 ng/ml. This POC SAW biosensor system can be further developed for diagnosing influenza.

TECHNOLOGY FEATURES & SPECIFICATIONS

This technology comprises a microfabrication process of a Surface Acoustic Wave (SAW) biosensor chip, a surface modification protocol for antibody immobilization, a sandwich immunoassay on microfabricated SAW biosensor chip for influenza viral protein detection, a polymer housing with microfluidic channel for bio sample delivery, and a point-of-care (POC) system for SAW biosensor signal reading. According to laboratory evaluation, the POC system can detect influenza viral protein with a sensitivity of 1 ng/ml. These biosensor platforms could overcome some of the challenges faced in conventional diagnosis techniques offering label-free assays with immediate results and employing small and user-friendly devices.

POTENTIAL APPLICATIONS

This Surface Acoustic Wave (SAW) biosensor based point-of-care (POC) system is developed specifically for influenza viral antigen detection. By immobilizing different antibodies, the biosensor platform can be modified for the detection of other disease-specific biomarkers. When multiple chips are integrated within a microfluidic channel for specimen processing and delivery, this POC system can be developed for multiplexed testing, which is simultaneous on-site detection of multiple analytes from a single specimen.

Benefits

Compared with other biosensing technologies, SAW biosensor has advantages of simpler operation, higher sensitivity, faster response, and lower cost. The point-of-care (POC) system developed is based on a SAW biosensor to bind and detect hemagglutinin (HA) of Influenza A H1N1 virus subtype. Based on laboratory evaluation, the POC system was capable of detecting HA antigen with sensitivity down to 1 ng/ml.