Identifying the authenticity in meat products is an important issue in food regulatory control for the determination of fraudulent replacement of higher commercial valued meat species by inferior, cheaper, or undesirable alternatives. A rapid, cost-effective examination of adulteration is a very critical issue for ethical requirements, specific food allergies, religious affairs, fraud, and malicious marketing practices in addition to safety, economic, religious (Halal testing), and legal concerns. Our rapid multiplex quantitative assay for meat speciation (beef, pork, chicken, sheep, dog, and horse) assay is novel and has multiple advantages over currently used meat identification assays.
TECHNOLOGY FEATURES & SPECIFICATIONS
The Rapid Multiplex Meat Speciation kit developed employs polymerase chain reaction (PCR) amplification in the presence of double stranded DNA binding dye followed by high resolution melting (HRM) technology. Detection of meat species namely beef, pork, lamb, duck or chicken is based on pre-determined melting point of PCR products. For example: a PCR product which melt at 70 degree indicates it is from beef, 74 degree from pork etc..
This method is rapid, sensitive, specific, cost-effective and does not require a probe.
It comprises of the following technologies:
Multiplex PCR optimization
Optimization of Melting Point of PCR products so that they can be distinguished from each other
Innovation method of detection based on pre-determined melting point of PCR products
Identitfication/authentication of meat (such as beef, pork, chicken, sheep, dog, and horse) and meat products (processed meat such as sausage, meatball etc.)
i) Advantages over traditional real-time PCR methods:
HRM-based methods provide greater flexibility as there is no requirement for fluorescently-labeled detection probes (such as TaqMan probes) - simplifying assay design.
ii) Specific and accurate detection method:
PCR products can be discriminated according to sequence, length, GC content, or strand complementarity, down to single base pair differences.
iii) Closed tube method - minimal contamination:
Cross-contamination is minimized as HRM is a closed-tube technique. Unlike other genotyping methods such as denaturing gradient gel electrophoresis (DGGE), HRM does not require the use of hazardous reagents such as acrylamide, formamide, and ethidium bromide.
iv) Cost-effective method:
HRM is cost effective as there is no requirement for fluorescently-labeled detection probes.