Production of β-Glucan Oligosaccharide by Ophiocordyceps Dipterigena BCC 2073 as Functional Ingredients for Industrial Applications

Technology Overview

A novel type of β-glucan of Ophiocordyceps dipterigena BCC 2073 has a size of 590-750 kDa which is 20 times bigger than yeast’s β-glucan. Its structure composes of a (1, 3)-β -D-glucan backbone, substituted at 0-6 with side chains of (1, 3)-β-D-pyranosyl unit. It is biocompatible, non-cytotoxic and a strong inducer of interleukin-8 (IL-8), a cytokine responsible for enhancing wound-healing process. Because of itsstructure and physical properties, it is possible for prebiotic usage. Its prebiotic properties in vitro andin vivo were studied, leading to new application of this β-glucan. Itcan be produced in submerged culture fermentation. The production yield of this β-glucan byO. dipterigena BCC 2073 is higher (60 g/L) thanother commercial β-glucans that have been reported. This mass production can reduce the cost of production and it can be applied in various applications. With the properties of non-cytotoxic, good gel-forming, highly moisturizing, high viscosity, well-adhesiveness and good tensile strength, this β-glucan can be used in cosmetic, food and feed, medical and pharmaceutical, and petroleum applications to replace high cost β-glucan from other sources. Thesize of the β-glucancan also be reduced by gamma-radiation andcustomised forspecific application. The targeted users and partners for collaboration are in the industries offood and feed, cosmetic, medical and pharmaceutical, and petroleum.

Technology Features & Specifications

The innovative aspectof this technology is the novelty of β-glucan production byOphiocordyceps dipterigena BCC 2073, with customisablesize appropriate for various applications.The higher production in pilot-scale fermentor is attractive for industrial applications. Unlike the processing steps for current β-glucans available in market,the technology produces β-glucan extracellularly anddoes nothavecomplicated downstream processes for its purification steps; thus resultingin a lower cost of production. The size reduction of this β-glucan by gamma-radiation also gave higher biological activities and immuno-enhancing propertieswith itssmaller molecular weight. The technology provideshighly-purified, small molecular weight, and different sizes of β-glucan oligosaccharides that can be used for variousapplications. The β-glucan from O. dipterigena BCC 2073 is an extracellular major product that is secreted out into the fermentation medium, which enables simpler downstream purification processes. The technology advantages are: (i) less purification steps, (ii) high yield of production, (iii) environmental-friendly as compared with existing technology (i.e. commercially-available β-glucanin the market useschemical processes forproduction). This is the first report of high-yield extracellular β-glucan production by O. dipterigena BCC 2073 obtained using low-cost media andappropriate fermentation processes, with subsequent β-glucan characterization and size reduction by gamma-radiation. Furthermore, the β-glucan production yieldis4.5-fold higher than that obtained after the optimization step, with shorter production time. The cost of media is $2 per kg of dried product, compared with glucose- and malt extract-based media.

Potential Applications

Animal feed:It can be used as a prebiotic in animals, which is beneficial to intestinal microflora that increased after oral fed of this β-glucan. The smaller sizes of β-glucan oligosaccharides enhanceimmune systems of animals, resultingin the health and growth rate of animal.This can reduce the useof antibiotics and chemical foranimals. Cosmetics:With the moisturising properties, this β-glucan can be potentially used in cosmetic application for wound-healing and anti-aging purposes. Functional food:The original size of β-glucan (750 Kda) is a highly-viscous solution that can lower the weight of mice, this can be used to addressobesity issue. The smaller sizes after gamma-radiation gave higher biological activities and immuno-modulating capacity. With its high viscosity, itcan also be used as gelling agents in food stabilizer application. Medical and pharmaceuticals:Epidermal cells gave higher titers of interleukin-8 after β-glucan applicationin which it stimulated the wound-healing capacity. Petroleum:It can be used as viscosifying agent for formulation of drilling mud, micellar flooding, and controlling mobility in enhanced oil recovery.

Customer Benefits

The benefitsfor the food and feed, cosmetics, medical and pharmaceutical, and petroleum businesses: (i) Lower cost of production (ii) High production yield (iii) Fermentation processes viaa microbial source which is environmental-friendly

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