Dr Sun Guang Wen is Lecturer at School of Applied Science, Republic Polytechnic with 15 years of experience in biochemistry, molecular and cell biology, immunology and microbiology. His current research interests include regulation of antibody response, production of recombinant antibodies, and development of quality assurance assays. In addition to education, Dr Sun is also an industrial consultant and provide advice in product development and quality assurance.
Prior to joining Republic Polytechnic, Dr Sun was Research Fellow at National University of Singapore and was involved in cutting edge research in emerging infectious diseases. His PhD and postdoctoral research were on the molecular and genetic basis of bacterial pathogenesis. His research experience also includes antibody engineering; gene knockout using CRISPR-Cas9; product quality analysis based on biochemical and molecular assays; and development of real-time PCR based human DNA detection method.
Dr Sun obtained his PhD in Microbiology and Immunology and BSc in Biochemistry from National University of Singapore.
Human DNA contamination is an issue in the process of manufacturing devices for molecular and genomic applications. Such devices need to be certified human DNA free for sensitive downstream processes. Therefore, there is a need to monitor human DNA residual level in factory surfaces and in final products. Dr Sun and his team identified a novel target for detection of human DNA. Based on this target, they developed a specific and sensitive, low-cost PCR based detection method for presence of human DNA. Compared to a commercial kit available in the market, this method is at least 500 times more sensitive. In addition, it can also be applied on forensic as well as environmental samples. Furthermore, the principle of the method can be adapted to detect other animal species for either identification or monitoring purposes.
- Life Sciences - Product Design & Development